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human gdf5 rhgdf5  (R&D Systems)


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    R&D Systems human gdf5 rhgdf5
    Figure 8. 1,25D blocks <t>rhGDF5</t> induced expression of BMP and IHH genes and p-SMAD1/5 and VDR expression is decreased in Hyp entheses by P14. (A) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10–8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subject- ed to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. (B) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10–8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. *P < 0.05 ver- sus WT; #P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. (C) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp, and C–/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.
    Human Gdf5 Rhgdf5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+gdf5+rhgdf5/pm37490334-182-25-30?v=R%26D+Systems
    Average 93 stars, based on 6 article reviews
    human gdf5 rhgdf5 - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "Impaired 1,25-dihydroxyvitamin D3 action underlies enthesopathy development in the Hyp mouse model of X-linked hypophosphatemia."

    Article Title: Impaired 1,25-dihydroxyvitamin D3 action underlies enthesopathy development in the Hyp mouse model of X-linked hypophosphatemia.

    Journal: JCI insight

    doi: 10.1172/jci.insight.163259

    Figure 8. 1,25D blocks rhGDF5 induced expression of BMP and IHH genes and p-SMAD1/5 and VDR expression is decreased in Hyp entheses by P14. (A) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10–8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subject- ed to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. (B) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10–8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. *P < 0.05 ver- sus WT; #P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. (C) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp, and C–/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.
    Figure Legend Snippet: Figure 8. 1,25D blocks rhGDF5 induced expression of BMP and IHH genes and p-SMAD1/5 and VDR expression is decreased in Hyp entheses by P14. (A) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10–8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subject- ed to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. (B) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10–8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. *P < 0.05 ver- sus WT; #P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. (C) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp, and C–/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.

    Techniques Used: Expressing, Incubation, Western Blot, Gene Expression



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    human gdf5 rhgdf5  (R&D Systems)
    93
    R&D Systems human gdf5 rhgdf5
    Figure 8. 1,25D blocks <t>rhGDF5</t> induced expression of BMP and IHH genes and p-SMAD1/5 and VDR expression is decreased in Hyp entheses by P14. (A) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10–8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subject- ed to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. (B) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10–8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. *P < 0.05 ver- sus WT; #P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. (C) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp, and C–/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.
    Human Gdf5 Rhgdf5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+gdf5+rhgdf5/pm37490334-182-25-30?v=R%26D+Systems
    Average 93 stars, based on 1 article reviews
    human gdf5 rhgdf5 - by Bioz Stars, 2026-08
    93/100 stars
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    Image Search Results


    Figure 8. 1,25D blocks rhGDF5 induced expression of BMP and IHH genes and p-SMAD1/5 and VDR expression is decreased in Hyp entheses by P14. (A) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10–8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subject- ed to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. (B) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10–8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. *P < 0.05 ver- sus WT; #P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. (C) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp, and C–/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.

    Journal: JCI insight

    Article Title: Impaired 1,25-dihydroxyvitamin D3 action underlies enthesopathy development in the Hyp mouse model of X-linked hypophosphatemia.

    doi: 10.1172/jci.insight.163259

    Figure Lengend Snippet: Figure 8. 1,25D blocks rhGDF5 induced expression of BMP and IHH genes and p-SMAD1/5 and VDR expression is decreased in Hyp entheses by P14. (A) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10–8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subject- ed to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. (B) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10–8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. *P < 0.05 ver- sus WT; #P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. (C) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp, and C–/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.

    Article Snippet: For Western blot analyses, chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with 1 × 10–8 M 1,25D prior to exposure to recombinant human GDF5 (rhGDF5) (100 ng/mL; R&D Systems) for 30 minutes.

    Techniques: Expressing, Incubation, Western Blot, Gene Expression