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human gdf5 rhgdf5  (R&D Systems)


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    Structured Review

    R&D Systems human gdf5 rhgdf5
    ( A ) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10 –8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL <t>rhGDF5</t> (for 30 minutes) and subjected to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. ( B ) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10 –8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. * P < 0.05 versus WT; # P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. ( C ) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp , and C –/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.
    Human Gdf5 Rhgdf5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human gdf5 rhgdf5/product/R&D Systems
    Average 92 stars, based on 12 article reviews
    human gdf5 rhgdf5 - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Impaired 1,25-dihydroxyvitamin D 3 action underlies enthesopathy development in the Hyp mouse model of X-linked hypophosphatemia"

    Article Title: Impaired 1,25-dihydroxyvitamin D 3 action underlies enthesopathy development in the Hyp mouse model of X-linked hypophosphatemia

    Journal: JCI Insight

    doi: 10.1172/jci.insight.163259

    ( A ) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10 –8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subjected to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. ( B ) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10 –8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. * P < 0.05 versus WT; # P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. ( C ) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp , and C –/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.
    Figure Legend Snippet: ( A ) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10 –8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subjected to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. ( B ) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10 –8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. * P < 0.05 versus WT; # P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. ( C ) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp , and C –/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.

    Techniques Used: Incubation, Western Blot, Expressing



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    human gdf5 rhgdf5  (R&D Systems)
    92
    R&D Systems human gdf5 rhgdf5
    ( A ) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10 –8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL <t>rhGDF5</t> (for 30 minutes) and subjected to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. ( B ) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10 –8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. * P < 0.05 versus WT; # P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. ( C ) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp , and C –/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.
    Human Gdf5 Rhgdf5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human gdf5 rhgdf5/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    human gdf5 rhgdf5 - by Bioz Stars, 2026-03
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10 –8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subjected to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. ( B ) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10 –8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. * P < 0.05 versus WT; # P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. ( C ) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp , and C –/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.

    Journal: JCI Insight

    Article Title: Impaired 1,25-dihydroxyvitamin D 3 action underlies enthesopathy development in the Hyp mouse model of X-linked hypophosphatemia

    doi: 10.1172/jci.insight.163259

    Figure Lengend Snippet: ( A ) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10 –8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subjected to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. ( B ) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10 –8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. * P < 0.05 versus WT; # P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. ( C ) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp , and C –/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.

    Article Snippet: For Western blot analyses, chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with 1 × 10 –8 M 1,25D prior to exposure to recombinant human GDF5 (rhGDF5) (100 ng/mL; R&D Systems) for 30 minutes.

    Techniques: Incubation, Western Blot, Expressing